Offer - Toxicological studies

List of standard toxicological studies:

In vitro, ex vivo, in vivo eye corrosion/irritation studies

Isolated chicken eye test method for identifying
I) chemicals inducing serious eye damage,

II) chemicals not requiring classification for eye irritation or serious eye damage
OECD Nr 438/ EU B.48

Short time exposure in vitro test method for identifying I) chemicals inducing serious eye damage

II) chemicals not requiring classification for eye irritation or serious eye damage.
OECD Nr 491/EU B.68
The test method used within the study on the model of reconstructed human cornea-like epithelium (RhCE), used for identifying chemicals not requiring classification and labelling for eye irritation or serious eye damage.
OECD Nr 492
Reconstructed human cornea-like epithelium (RHCE) test method for eye hazard identification
OECD Nr 492B
Acute eye irritation/ eye corrosion test
OECD Nr 405/EU B.5
In vitro, ex vivo, in vivo skin corrosion/irritation studies
In vitro skin corrosion: transcutaneous electrical resistance test (TER).
OECD Nr 430/ EU B.40
In vitro skin corrosion: reconstructed human epidermis (RHE) test method
OECD Nr 431/ EU B.40
In vitro skin irritation. The test on the model of reconstructed human epidermis
OECD Nr 439/ EU B.46
Acute skin irritation/ corrosion test.
OECD Nr 404/ EU B.4
Biological evaluation of medical devices – Test for skin irritation in vitro.
ISO 10993-23
In vitro, in chemico, in vivo skin sensitisation studies
Skin sensitisation: local lymph node assay (LLNA): BrdU-ELISA.
OECD Nr 442B/EU B.51
In chemico skin sensitisation, direct peptide reactivity assay (DPRA).
OECD Nr 442C/ EU B.59
In vitro skin sensitisation: the test method with use of ARE-Nrf2 Luciferase
OECD Nr 442D/ EU B.60
In vitro skin sensitisation: Human cell line activation test (H-CLAT).
OECD Nr 442E/ EU B.71
Skin sensitisation study
OECD Nr 406/ EU B.6
Delayed hypersensitivity assessment test using the closed-patch method (Buehler Test).
ISO 10993-10
In vitro cytotoxicity studies
In vitro cytotoxicity test.
ISO 10993-5
In vitro phototoxicity
Phototoxicity - In vitro 3T3 NRU phototoxicity test.
OECD Nr 432
In vitro Phototoxicity - Reconstructed Human Epidermis Phototoxicity test method
OECD 498
In vitro, in vivo studies identifying endocrine-disrupting substances
Stably Transfected Human Estrogen Receptor-α Transactivation Assay for Detection of Estrogenic Agonist and antagonist Activity of Chemicals using the hERα-HeLa-9903 cell line.
OECD Nr 455

Uterotrophic Bioassay in Rodents

A short-term screening test for oestrogenic properties.
OECD Nr 440/ B.54
Hershberger bioassay in rats. Short-term screening test of (anti-) androgenic properties
OECD Nr 441 B.55
In vitro, in vivo acute toxicity
Use of the 3T3 neutral red uptake cytotoxicity test to estimate starting doses for acute oral systemic toxicity tests.
OECD Series on Testing and Assessment No. 129 (2010)

Acute oral toxicity test.
OECD Nr 420/ EU B.1.BIS
Acute oral/ intraperitoneal/ subcutaneous/ intravascular toxicity test - acute toxic class method.
OECD Nr 423/ EU B.1.TRIS
Acute dermal toxicity test.
OECD Nr 402/ EU B.3
Acute inhalation toxicity test.
OECD Nr 403
Acute inhalation toxicity – acute toxic class method.
OECD Nr 436/ EU B.52
Maximum tolerated dose test (MTD).
Single dose toxicity study in rodents.
ISO 10993-11
In vivo subacute, subchronic and chronic studies
Dose range finding  test (DRF
Repeated dose 28-day oral/ intraperitoneal/ subcutaneous toxicity study in rodents
OECD Nr 407/ EU B.7
Repeated dose dermal toxicity: 21/ 28 day study
OECD Nr 410/ EU B.9
Repeated dose 90-day oral toxicity study in rodents
OECD Nr 408/ EU B.26
Subchronic dermal toxicity: 90-day study
OECD Nr 411/ EU B.28
Chronic toxicity studies
OECD Nr 452/ EU B.30
Combined chronic toxicity/ carcinogenicity studies
OECD Nr 453/ EU B.33
In vivo neurotoxicity studies
Neurotoxicity study in rodents.
OECD Nr 424/ EU B.43
In vitro developmental and reproduction studies
Prenatal developmental toxicity study
OECD Nr 414/ EU B.31
Reproduction/ developmental toxicity screening test.
OECD Nr 421
Combined repeated dose toxicity study with the reproduction/ developmental toxicity screening test.
OECD Nr 422
Extended one-generation reproductive toxicity study in rats
OECD Nr 443/ EU B.56
Two-generation reproduction toxicity study
OECD Nr 416/ EU B.35
In vitro, in vivo genotoxicity studies
Bacterial reverse mutation test (AMES test, microplate format)
w oparciu o OECD Nr 471/ EU B.13/14
In vitro micronucleus test on the mammalian cell line
OECD Nr 487/ EU B.49
In vitro mammalian cell gene mutation tests using the HPRT genes
OECD Nr 476/ EU B.17
In vivo mammalian erythrocyte micronucleus test
OECD Nr 474/ EU B.12
In vivo mammalian cell gene mutation test using the thymidine kinase gene
OECD Nr 490/ EU B.67
In Vivo Mammalian Alkaline Comet Assay
OECD Nr. 489
In vivo carcinogenicity studies
Carcinogenicity studies
OECD Nr 451/ EU B.32
Combined chronic toxicity/ carcinogenicity studies
OECD Nr 453/ EU B.33
In vivo local tolerance studies
Toxicokinetics/pharmacokinetics studies
Toxicokinetics/pharmacokinetics study
OECD Nr 417/ EU B.436/ ICH S3A
Other services
Toxicological studies conducted according to the procedures agreed with the customer
Haematological studies
Biochemical studies
Hormone identification
Tissue and organ collection
Preparing histopathology slides and their scanning

On request, other tests can be developed and performed.
For any additional questions contact us at or

Toxicology Research Group

Toxicological tests performed at the IPO can be characterized as follows:
  • they allow a toxicological evaluation of chemical substances contained in pharmaceutical products, veterinary medicinal products, plant protection products, food and feed additives, industrial chemical substances, etc.,
  • they make it possible to elaborate models which allow one to observe chemical substances in the body and to explain the mechanisms of various toxic effects caused by them,
  • they serve as a basis for a routine evaluation of new chemical compounds,
  • their results are used to register and license industrial chemical substances in compliance with specific regulations.